At Neurocrine Biosciences, we pride ourselves on having a strong, inclusive, and positive culture based on our shared purpose and values. We know what it takes to be great, and we are as passionate about our people as we are about our purpose - to relieve suffering for people with great needs, but few options.

Neurocrine Biosciences is a neuroscience-focused, biopharmaceutical company with a simple purpose: to relieve suffering for people with great needs, but few options. We are dedicated to discovering and developing life-changing treatments for patients with under-addressed neurological, endocrine and psychiatric disorders. The company's diverse portfolio includes FDA-approved treatments for tardive dyskinesia, Parkinson's disease, endometriosis* and uterine fibroids*, as well as clinical programs in multiple therapeutic areas. For three decades, we have applied our unique insight into neuroscience and the interconnections between brain and body systems to treat complex conditions. We relentlessly pursue medicines to ease the burden of debilitating diseases and disorders, because you deserve brave science. *in collaboration with AbbVie

About the Role:

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• Designs and performs experiments to support scientific activities and project goals involving cell including primary human leukocytes, mouse splenocytes, iPCS derived microglia cells and biochemical assays

• Generates data and works with complex assay system, with a strong understanding of advanced data analysis and statistical tests

• Responsible for advanced cell-based assay development, including human whole blood, human and rodent primary cells, coculture and ex vivo tissues

• High skills in isolating primary immune cells from various organs in experimental animal models. Knowing how to set up the in vitro assays to understand the cellular functions of innate and adaptive immune cells as well as the cell-cell interaction

• Extensive experience in human PBMC isolation, lymphocyte proliferation, activation, cytokine secretion and differentiation assays

• High skills for knowing how to perform multiple color flow cytometry assay including intracellular staining and prepare for cell sorting as well as flowcytometry data analysis

• Basic molecular biology skills including mRNA isolation, TagMan, NanoString and scRNAseq experience is highly desirable

• Extensive in vivo experience of autoimmune diseases and other inflammatory diseases is a plus. Also knowing how to develop animal disease models

• Maintains and updates SOP's and writes data summaries for experiments conducted

• Provides training and/or supervision to junior staff, as needed

• Other duties as assigned

• BS/BA degree and 3-5+ years of relevant experience OR

• MS/MA degree and 2+ years of relevant experience

• Advanced tissue culture techniques, including knowledge of human PBMC isolation, mouse splenocyte, iPCS and precursor cell handling and differentiation as well as hematopoietic cell differentiation

• Animal handling and in vivo animal model development and testing

• Working knowledge of NGS technologies, their implementation, and data analysis & relevant statistical tests

• Gene manipulation techniques, including cloning, and use of gene expression manipulation technologies (ASO, siRNA, miRNA, AAV transduction)

• Tissue handling techniques for detection of gene and protein expression, including fixation, sectioning, IHC, histological staining, ISH

• Demonstrates solid level of understanding project / group goals and methods

• Consistently recognizes anomalous and inconsistent results and interprets experimental outcomes

• Able to explain the process behind the data and implications of the results

• Strong knowledge of one or more scientific disciplines especially Immunology

• Strong knowledge of scientific principles, methods and techniques.

• Strong knowledge and demonstrated ability working with a variety of laboratory equipment/tools

• High skills on handling animals and various procedures including IV, SC, IP injection etc

• Ability to work as part of a team; may train lower levels

• Excellent computer skills

• Strong communications, problem-solving, analytical thinking skills

• Detail oriented yet can see broader picture of scientific impact on team

• Ability to meet multiple deadlines, with a high degree of accuracy and efficiency

• Strong project management skills

• Ability to meet multiple deadlines, with a high degree of accuracy and efficiency

Neurocrine Biosciences is an EEO/AA/Disability/Vets employer.

We are committed to building a diverse, equitable, and inclusive workplace, and we recognize there are a variety of ways to meet our requirements. We are looking for the best candidate for the job and encourage you to apply even if your experience or qualifications don't line up to exactly what we have outlined in the job description.

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